Researchers are becoming increasingly aware that quantitative systemic studies demand for methods that do not/or only minimally disturb the cells under investigation. Consequently, recent technological break troughs have seen several new methods for tailored manipulation of genomic DNA inside cells, necessary to achieve endogenous expression levels of labeled proteins from their original genomic context.
In addition, methods have to be developed that permit access to specific parameters, such as the ones underlying protein dynamics, by means of generic approaches. Only this permits the necessary standardization and throughput for systemic studies.
Our lab is active in two fields related to methods development.