Objective comparison
Leica HCXPlApo
100 X
1.40
Image stacks taken on a Leica DMRXA with HCXPlApo 100x 1.4, a 1024x1344 (6.45 μm) pixel CCD camera (Orca-ER, Hamamatsu), and 470/40, 560/40 excitation and 525/50, 645/75 emission filters (all Chroma). Voxel size is 65x65x65 nm.Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).
Nikon Apo lambda
100 X
1.45
Image stacks taken on a Nikon Ti-E with PlApo 100x 1.45, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).
Nikon ApoTIRF
100 X
1.49
Image stacks taken on a Nikon Ti-E with ApoTIRF 100x 1.49, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).
