A tool to probe your microscope's performance

PSFj analyzes a stack of images of fluorescent beads in order to calculate the resolution of your microscope at different positions of your fields of view. PSFj is written in JAVA and compatible with Windows, MacOS X and Linux.

Download now !

Access paper @ Nature Methods

Objective comparison

Leica HCXPlApo 100 X 1.40
Image stacks taken on a Leica DMRXA with HCXPlApo 100x 1.4, a 1024x1344 (6.45 μm) pixel CCD camera (Orca-ER, Hamamatsu), and 470/40, 560/40 excitation and 525/50, 645/75 emission filters (all Chroma). Voxel size is 65x65x65 nm.Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).
Nikon Apo lambda 100 X 1.45
Image stacks taken on a Nikon Ti-E with PlApo 100x 1.45, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).
Nikon ApoTIRF 100 X 1.49
Image stacks taken on a Nikon Ti-E with ApoTIRF 100x 1.49, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

More objectives...