ObjectivesPSFj

Leica HCXPlApo 100 X 1.40

Image stacks taken on a Leica DMRXA with HCXPlApo 100x 1.4, a 1024x1344 (6.45 μm) pixel CCD camera (Orca-ER, Hamamatsu), and 470/40, 560/40 excitation and 525/50, 645/75 emission filters (all Chroma). Voxel size is 65x65x65 nm.Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Nikon Apo lambda 100 X 1.45

Image stacks taken on a Nikon Ti-E with PlApo 100x 1.45, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Nikon ApoTIRF 100 X 1.49

Image stacks taken on a Nikon Ti-E with ApoTIRF 100x 1.49, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Nikon ApoTIRF 60 X 1.49

Image stacks taken on a Nikon Ti-E with ApoTIRF 60x 1.49, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 108x108x108 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Nikon PlApoW 60 X 1.27

Image stacks taken on a Nikon Ti-E with PlApoW 60x 1.27, a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 108x108x108 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Nikon PlApoW 25 X 1.10

Image stacks taken on a Nikon Ti-E with PlApoW 25x 1.1 (total magnification was set to 25x*1.5x=37.5x), a 2048x2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 173x173x173 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Olympus PlApo 100 X 1.40

Image stacks taken on a Olympus xcellence with PlApo 100x 1.45, a 1024x1344 pixel (6.45 μm) CCD camera (Orca-R2, Hamamatsu), and a GFP-mCherry ET filter set (Chroma). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Olympus PlApoN 60 X 1.42

Image stacks taken on a DeltaVision Elite (Applied Precision) with PlApoN 60x 1.42 (Olympus), a 2048x2048 pixel (6.5 μm) sCMOS camera (pco.edge 4.2, PCO), and 475/28, 575/25 excitation and 525/50, 624/40 emission filters (all from Semrock). Voxel size is 108x108x108 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Olympus UPlSApo 100 X 1.40

Image stacks taken on a DeltaVision Elite (Applied Precision) with UPlSApo 100x 1.4 (Olympus), a 2048x2048 pixel (6.5 μm) sCMOS camera (pco.edge 4.2, PCO), and 475/28, 575/25 excitation and 525/50, 624/40 emission filters (all from Semrock). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Olympus UPlSApo 60 X 1.35

Image stacks taken on a Olympus xcellence with UPlSApo 60x 1.35, a 1024x1344 pixel (6.45 μm) CCD camera (Orca-R2, Hamamatsu), and a GFP-mCherry ET filter set (Chroma). Voxel size is 108x108x108 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Zeiss PlApo 100 X 1.40

Image stacks taken on a Zeiss Axio Observer Z1 with PlanApo 100x 1.4, a 1040x1388 pixel (6.45 μm) CCD camera (AxioCamMRm, Zeiss), and a GFP and DsRed filter set (38HE and 43HE, respectively, both Zeiss). Voxel size is 65x65x65 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).

Zeiss PlApo 63 X 1.40

Image stacks taken on a Zeiss Axio Observer Z1 with PlanApo 63x 1.4, a 1040x1388 pixel (6.45 μm) CCD camera (AxioCamMRm, Zeiss), and a GFP and DsRed filter set (38HE and 43HE, respectively, both Zeiss). Voxel size is 102x102x102 nm. Beads were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes).