Here is the list of images used for the publication.

• Nikon ApoTIRF 100x 1.49 1.1 GBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu) and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Nikon ApoTIRF 60x 1.49 (Single channel) 2.4GBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu) and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 108x108x108 nm. Beads imaged were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes). The file contains sub-folders with image stacks taken on different days.
• Nikon ApoTIRF 60x 1.49 (Dual channel) 640MBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu) and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 108x108x108 nm. Beads imaged were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Nikon ApoTIRF 60x 1.49 (Objective & microscope comparison) 1.1 GBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu) and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 108x108x108 nm. Beads imaged were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes). The file contains sub-folders with image stacks taken for three identical objectives on one microscope (“07-08-13_objective comparison”), and for one objective on two identical microscopes (“07-30-13_microscope comparison”).
• Leica HCXPlApo 100x 1.4 240MBImage stacks taken on a Leica DMRXA with a 1024×1344 (6.45 μm) pixel CCD camera (Orca-ER, Hamamatsu), and 470/40, 560/40 excitation and 525/50, 645/75 emission filters (all Chroma). Voxel size is 65x65x65 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Nikon PlApo 100x 1.45 (Single channel part 1) 1.2GBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads imaged were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes). The file contains sub-folders with image stacks taken on different days.
• Nikon PlApo 100x 1.45 (Single channel part 2) 2.2GBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads imaged were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes). The file contains sub-folders with image stacks taken on different days.
• Nikon PlApo 100x 1.45 (Dual channel) 900MBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 65x65x65 nm. Beads imaged were 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes)
• Olympus PlApoN 60x 1.42 920MBImage stacks taken on a DeltaVision Elite (Applied Precision) with a 2048×2048 pixel (6.5 μm) sCMOS camera (pco.edge 4.2, PCO), and 475/28, 575/25 excitation and 525/50, 624/40 emission filters (all from Semrock). Voxel size is 108x108x108 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Nikon PlApoW 25x 1.1 570MBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). The total magnification was set to 25x*1.5x=37.5x. Voxel size is 173x173x173 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Nikon PlApoW 60x 1.27 830MBImage stacks taken on a Nikon Ti-E with a 2048×2048 pixel (6.5 μm) sCMOS camera (Flash4, Hamamatsu), and 470/40, 575/25 excitation and 525/50, 632/60 emission filters (all from Chroma except 575/25, which was from Semrock). Voxel size is 108x108x108 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Olympus PlApo 100x 1.45 190MBImage stacks taken on a Olympus xcellence with a 1024×1344 pixel (6.45 μm) CCD camera (Orca-R2, Hamamatsu), and a GFP-mCherry ET filter set (Chroma). Voxel size is 65x65x65 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Olympus UPlSApo 100x 1.4 1.2GBImage stacks taken on a DeltaVision Elite (Applied Precision) with a 2048×2048 pixel (6.5 μm) sCMOS camera (pco.edge 4.2, PCO), and 475/28, 575/25 excitation and 525/50, 624/40 emission filters (all from Semrock). Voxel size is 65x65x65 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Olympus UPlSApo 60x 1.35 220MBImage stacks taken on a Olympus xcellence with a 1024×1344 pixel (6.45 μm) CCD camera (Orca-R2, Hamamatsu), and a GFP-mCherry ET filter set (Chroma). Voxel size is 108x108x108 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Zeiss PlanApo 100x 1.4 190MBImage stacks taken on a Zeiss Axio Observer Z1 with a 1040×1388 pixel (6.45 μm) CCD camera (AxioCamMRm, Zeiss), and a GFP and DsRed filter set (38HE and 43HE, respectively, both Zeiss). Voxel size is 65x65x65 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.
• Zeiss PlanApo 63x 1.4 200MBImage stacks taken on a Zeiss Axio Observer Z1 with a 1040×1388 pixel (6.45 μm) CCD camera (AxioCamMRm, Zeiss), and a GFP and DsRed filter set (38HE and 43HE, respectively, both Zeiss). Voxel size is 102x102x102 nm. Beads imaged in “single channel” and “dual channel” stacks were 0.175 μm diameter (green) single-color beads (PS-speck, Kit P-7220, Molecular Probes) and 0.220 μm diameter multicolor beads (TetraSpeck, T-7280, Molecular Probes) respectively.