Major Publications

All reserach papers (no reviews) with Michael Knop being (co-)corresponding or (co-)first author

Timer-based proteomic profiling of the ubiquitin-proteasome system reveals a substrate receptor of the GID ubiquitin ligase.

Kong KE, Fischer B, Meurer M, Kats I, Li Z, Rühle F, Barry JD, Kirrmaier D, Chevyreva V, San Luis BJ, Costanzo M, Huber W, Andrews BJ, Boone C, Knop M, Khmelinskii A.

Mol Cell. 2021 Jun 3;81(11):2460-2476.e11. doi: 10.1016/j.molcel.2021.04.018. Epub 2021 May 10.

PMID: 33974913 Free PMC article.

Up-regulation of ubiquitin-proteasome activity upon loss of NatA-dependent N-terminal acetylation.

Kats I, Reinbold C, Kschonsak M, Khmelinskii A, Armbruster L, Ruppert T, Knop M.

Life Sci Alliance. 2021 Nov 11;5(2):e202000730. doi: 10.26508/lsa.202000730. Print 2022 Feb.

PMID: 34764209 Free PMC article.

Sex without crossing over in the yeast Saccharomycodes ludwigii.

Papaioannou IA, Dutreux F, Peltier FA, Maekawa H, Delhomme N, Bardhan A, Friedrich A, Schacherer J, Knop M.

Genome Biol. 2021 Nov 3;22(1):303. doi: 10.1186/s13059-021-02521-w.

PMID: 34732243 Free PMC article.

Single-Color Fluorescence Lifetime Cross-Correlation Spectroscopy In Vivo.

Štefl M, Herbst K, Rübsam M, Benda A, Knop M.

Biophys J. 2020 Oct 6;119(7):1359-1370. doi: 10.1016/j.bpj.2020.06.039. Epub 2020 Aug 20.

PMID: 32919495 Free PMC article.

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples.

Dao Thi VL, Herbst K, Boerner K, Meurer M, Kremer LP, Kirrmaier D, Freistaedter A, Papagiannidis D, Galmozzi C, Stanifer ML, Boulant S, Klein S, Chlanda P, Khalid D, Barreto Miranda I, Schnitzler P, Kräusslich HG, Knop M, Anders S.

Sci Transl Med. 2020 Aug 12;12(556):eabc7075. doi: 10.1126/scitranslmed.abc7075. Epub 2020 Jul 27.

PMID: 32719001 Free PMC article.

CRISPR-Cas12a-assisted PCR tagging of mammalian genes.

Fueller J, Herbst K, Meurer M, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller BC, Pereira G, Lemberg MK, Knop M.

J Cell Biol. 2020 Jun 1;219(6):e201910210. doi: 10.1083/jcb.201910210.

PMID: 32406907 Free PMC article.

Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast.

Buchmuller BC, Herbst K, Meurer M, Kirrmaier D, Sass E, Levy ED, Knop M.

Nat Commun. 2019 Jul 4;10(1):2960. doi: 10.1038/s41467-019-10816-7.

PMID: 31273196 Free PMC article.

Self-Learning Microfluidic Platform for Single-Cell Imaging and Classification in Flow.

Constantinou I, Jendrusch M, Aspert T, Görlitz F, Schulze A, Charvin G, Knop M.

Micromachines (Basel). 2019 May 9;10(5):311. doi: 10.3390/mi10050311.

PMID: 31075890 Free PMC article.

Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis.

Durrieu L, Kirrmaier D, Schneidt T, Kats I, Raghavan S, Hufnagel L, Saunders TE, Knop M.

Mol Syst Biol. 2018 Sep 4;14(9):e8355. doi: 10.15252/msb.20188355.

PMID: 30181144 Free PMC article.

10.

Genome-wide C-SWAT library for high-throughput yeast genome tagging.

Meurer M, Duan Y, Sass E, Kats I, Herbst K, Buchmuller BC, Dederer V, Huber F, Kirrmaier D, Štefl M, Van Laer K, Dick TP, Lemberg MK, Khmelinskii A, Levy ED, Knop M.

Nat Methods. 2018 Aug;15(8):598-600. doi: 10.1038/s41592-018-0045-8. Epub 2018 Jul 9.

PMID: 29988096

11.

Mapping Degradation Signals and Pathways in a Eukaryotic N-terminome.

Kats I, Khmelinskii A, Kschonsak M, Huber F, Knieß RA, Bartosik A, Knop M.

Mol Cell. 2018 May 3;70(3):488-501.e5. doi: 10.1016/j.molcel.2018.03.033.

PMID: 29727619

12.

Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities.

Bunina D, Štefl M, Huber F, Khmelinskii A, Meurer M, Barry JD, Kats I, Kirrmaier D, Huber W, Knop M.

Nucleic Acids Res. 2017 Nov 2;45(19):11144-11158. doi: 10.1093/nar/gkx737.

PMID: 28977638 Free PMC article.

13.

The regulatable MAL32 promoter in Saccharomyces cerevisiae: characteristics and tools to facilitate its use.

Meurer M, Chevyreva V, Cerulus B, Knop M.

Yeast. 2017 Jan;34(1):39-49. doi: 10.1002/yea.3214. Epub 2016 Nov 23.

PMID: 27714848

14.

πSPIM: high NA high resolution isotropic light-sheet imaging in cell culture dishes.

Theer P, Dragneva D, Knop M.

Sci Rep. 2016 Sep 13;6:32880. doi: 10.1038/srep32880.

PMID: 27619647 Free PMC article.

15.

Protein Abundance Control by Non-coding Antisense Transcription.

Huber F, Bunina D, Gupta I, Khmelinskii A, Meurer M, Theer P, Steinmetz LM, Knop M.

Cell Rep. 2016 Jun 21;15(12):2625-36. doi: 10.1016/j.celrep.2016.05.043. Epub 2016 Jun 9.

PMID: 27292640 Free PMC article.

16.

One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.

Yofe I, Weill U, Meurer M, Chuartzman S, Zalckvar E, Goldman O, Ben-Dor S, Schütze C, Wiedemann N, Knop M, Khmelinskii A, Schuldiner M.

Nat Methods. 2016 Apr;13(4):371-378. doi: 10.1038/nmeth.3795. Epub 2016 Feb 29.

PMID: 26928762 Free PMC article.

17.

Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers.

Khmelinskii A, Meurer M, Ho CT, Besenbeck B, Füller J, Lemberg MK, Bukau B, Mogk A, Knop M.

Mol Biol Cell. 2016 Jan 15;27(2):360-70. doi: 10.1091/mbc.E15-07-0525. Epub 2015 Nov 25.

PMID: 26609072 Free PMC article.

18.

Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein.

Guan Y, Meurer M, Raghavan S, Rebane A, Lindquist JR, Santos S, Kats I, Davidson MW, Mazitschek R, Hughes TE, Drobizhev M, Knop M, Shah JV.

Mol Biol Cell. 2015 Jun 1;26(11):2054-66. doi: 10.1091/mbc.E14-10-1473. Epub 2015 Apr 15.

PMID: 25877871 Free PMC article.

19.

Protein quality control at the inner nuclear membrane.

Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus DJ, Le Dez G, Brossard A, Gunnarsson A, Barry JD, Meurer M, Kirrmaier D, Boone C, Huber W, Rabut G, Ljungdahl PO, Knop M.

Nature. 2014 Dec 18;516(7531):410-3. doi: 10.1038/nature14096.

PMID: 25519137 Free PMC article.

20.

PCR Duplication: A One-Step Cloning-Free Method to Generate Duplicated Chromosomal Loci and Interference-Free Expression Reporters in Yeast.

Huber F, Meurer M, Bunina D, Kats I, Maeder CI, Stefl M, Mongis C, Knop M.

PLoS One. 2014 Dec 10;9(12):e114590. doi: 10.1371/journal.pone.0114590. eCollection 2014.

PMID: 25493941 Free PMC article.

21.

PSFj: know your fluorescence microscope.

Theer P, Mongis C, Knop M.

Nat Methods. 2014 Oct;11(10):981-2. doi: 10.1038/nmeth.3102.

PMID: 25264772 No abstract available.

22.

Analysis of protein dynamics with tandem fluorescent protein timers.

Khmelinskii A, Knop M.

Methods Mol Biol. 2014;1174:195-210. doi: 10.1007/978-1-4939-0944-5_13.

PMID: 24947383

23.

Analytical model for macromolecular partitioning during yeast cell division.

Kinkhabwala A, Khmelinskii A, Knop M.

BMC Biophys. 2014 Sep 23;7:10. doi: 10.1186/s13628-014-0010-6. eCollection 2014.

PMID: 25737777 Free PMC article.

24.

Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.

Gibeaux R, Politi AZ, Nédélec F, Antony C, Knop M.

Genes Dev. 2013 Feb 1;27(3):335-49. doi: 10.1101/gad.206318.112.

PMID: 23388829 Free PMC article.

25.

Tandem fluorescent protein timers for in vivo analysis of protein dynamics.

Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E, Knop M.

Nat Biotechnol. 2012 Jun 24;30(7):708-14. doi: 10.1038/nbt.2281.

PMID: 22729030

26.

Seamless gene tagging by endonuclease-driven homologous recombination.

Khmelinskii A, Meurer M, Duishoev N, Delhomme N, Knop M.

PLoS One. 2011;6(8):e23794. doi: 10.1371/journal.pone.0023794. Epub 2011 Aug 22.

PMID: 21915245 Free PMC article.

27.

Quantitative fluorescence imaging of protein diffusion and interaction in living cells.

Capoulade J, Wachsmuth M, Hufnagel L, Knop M.

Nat Biotechnol. 2011 Aug 7;29(9):835-9. doi: 10.1038/nbt.1928.

PMID: 21822256

28.

Genomic promoter replacement cassettes to alter gene expression in the yeast Saccharomyces cerevisiae.

Kaufmann A, Knop M.

Methods Mol Biol. 2011;765:275-94. doi: 10.1007/978-1-61779-197-0_16.

PMID: 21815098

29.

Segregation of yeast nuclear pores.

Khmelinskii A, Keller PJ, Lorenz H, Schiebel E, Knop M.

Nature. 2010 Jul 22;466(7305):E1. doi: 10.1038/nature09255.

PMID: 20651645

30.

Evolution of mutational robustness in the yeast genome: a link to essential genes and meiotic recombination hotspots.

Keller PJ, Knop M.

PLoS Genet. 2009 Jun;5(6):e1000533. doi: 10.1371/journal.pgen.1000533. Epub 2009 Jun 26.

PMID: 19557188 Free PMC article.

31.

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron.

Taxis C, Stier G, Spadaccini R, Knop M.

Mol Syst Biol. 2009;5:267. doi: 10.1038/msb.2009.25. Epub 2009 Apr 28.

PMID: 19401679 Free PMC article.

32.

The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis.

Maier P, Rathfelder N, Maeder CI, Colombelli J, Stelzer EH, Knop M.

EMBO J. 2008 Sep 17;27(18):2363-74. doi: 10.1038/emboj.2008.168. Epub 2008 Aug 28.

PMID: 18756268 Free PMC article.

33.

Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling.

Maeder CI, Hink MA, Kinkhabwala A, Mayr R, Bastiaens PI, Knop M.

Nat Cell Biol. 2007 Nov;9(11):1319-26. doi: 10.1038/ncb1652. Epub 2007 Oct 21.

PMID: 17952059

34.

Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane.

Maier P, Rathfelder N, Finkbeiner MG, Taxis C, Mazza M, Le Panse S, Haguenauer-Tsapis R, Knop M.

EMBO J. 2007 Apr 4;26(7):1843-52. doi: 10.1038/sj.emboj.7601621. Epub 2007 Mar 8.

PMID: 17347652 Free PMC article.

35.

Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast.

Taxis C, Maeder C, Reber S, Rathfelder N, Miura K, Greger K, Stelzer EH, Knop M.

Traffic. 2006 Dec;7(12):1628-42. doi: 10.1111/j.1600-0854.2006.00496.x.

PMID: 17118118

36.

Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis.

Gordon O, Taxis C, Keller PJ, Benjak A, Stelzer EH, Simchen G, Knop M.

EMBO J. 2006 Aug 23;25(16):3856-68. doi: 10.1038/sj.emboj.7601254. Epub 2006 Aug 3.

PMID: 16888627 Free PMC article.

37.

System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae.

Taxis C, Knop M.

Biotechniques. 2006 Jan;40(1):73-8. doi: 10.2144/000112040.

PMID: 16454043

38.

Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system.

Taxis C, Keller P, Kavagiou Z, Jensen LJ, Colombelli J, Bork P, Stelzer EH, Knop M.

J Cell Biol. 2005 Nov 21;171(4):627-40. doi: 10.1083/jcb.200507168. Epub 2005 Nov 14.

PMID: 16286509 Free PMC article.

39.

Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis.

Riedel CG, Mazza M, Maier P, Körner R, Knop M.

J Biol Chem. 2005 Nov 11;280(45):37846-52. doi: 10.1074/jbc.M504244200. Epub 2005 Sep 7.

PMID: 16148007

40.

Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast.

Knop M, Miller KJ, Mazza M, Feng D, Weber M, Keränen S, Jäntti J.

Mol Biol Cell. 2005 Oct;16(10):4543-56. doi: 10.1091/mbc.e05-03-0243. Epub 2005 Jul 19.

PMID: 16030256 Free PMC article.

41.

A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.

Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M.

Yeast. 2004 Aug;21(11):947-62. doi: 10.1002/yea.1142.

PMID: 15334558

42.

Improved version of the red fluorescent protein (drFP583/DsRed/RFP).

Knop M, Barr F, Riedel CG, Heckel T, Reichel C.

Biotechniques. 2002 Sep;33(3):592, 594, 596-8 passim. doi: 10.2144/02333rr02.

PMID: 12238769

43.

Prospore membrane formation linked to the leading edge protein (LEP) coat assembly.

Moreno-Borchart AC, Strasser K, Finkbeiner MG, Shevchenko A, Shevchenko A, Knop M.

EMBO J. 2001 Dec 17;20(24):6946-57. doi: 10.1093/emboj/20.24.6946.

PMID: 11742972 Free PMC article.

44.

Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis.

Knop M, Strasser K.

EMBO J. 2000 Jul 17;19(14):3657-67. doi: 10.1093/emboj/19.14.3657.

PMID: 10899120 Free PMC article.

45.

Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines.

Knop M, Siegers K, Pereira G, Zachariae W, Winsor B, Nasmyth K, Schiebel E.

Yeast. 1999 Jul;15(10B):963-72. doi: 10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W.

PMID: 10407276

46.

Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation.

Knop M, Schiebel E.

EMBO J. 1998 Jul 15;17(14):3952-67. doi: 10.1093/emboj/17.14.3952.

PMID: 9670012 Free PMC article.

47.

Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p.

Knop M, Schiebel E.

EMBO J. 1997 Dec 1;16(23):6985-95. doi: 10.1093/emboj/16.23.6985.

PMID: 9384578 Free PMC article.

48.

The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication.

Knop M, Pereira G, Geissler S, Grein K, Schiebel E.

EMBO J. 1997 Apr 1;16(7):1550-64. doi: 10.1093/emboj/16.7.1550.

PMID: 9130700 Free PMC article.

49.

N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast.

Knop M, Hauser N, Wolf DH.

Yeast. 1996 Sep 30;12(12):1229-38. doi: 10.1002/(sici)1097-0061(19960930)12:12<1229::aid-yea15>3.0.co;2-h.

PMID: 8905927

50.

Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast.

Knop M, Finger A, Braun T, Hellmuth K, Wolf DH.

EMBO J. 1996 Feb 15;15(4):753-63.

PMID: 8631297 Free PMC article.

51.

Analysis of two mutated vacuolar proteins reveals a degradation pathway in the endoplasmic reticulum or a related compartment of yeast.

Finger A, Knop M, Wolf DH.

Eur J Biochem. 1993 Dec 1;218(2):565-74. doi: 10.1111/j.1432-1033.1993.tb18410.x.

PMID: 8269947

52.

Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy.

Pereira G, Grueneberg U, Knop M, Schiebel E.

EMBO J. 1999 Aug 2;18(15):4180-95. doi: 10.1093/emboj/18.15.4180.

PMID: 10428957 Free PMC article.

53.

Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication.

Elliott S, Knop M, Schlenstedt G, Schiebel E.

Proc Natl Acad Sci U S A. 1999 May 25;96(11):6205-10. doi: 10.1073/pnas.96.11.6205.

PMID: 10339566 Free PMC article.