Research highlights
past and present
this side is under construction
+ tandem fluorescent timer proteins (tFTs)
"measure protein age and protein turnover in space and time"
+ Protein quality control in the nucleus
Removal of mis-targeted proteins from the inner nuclear membrane

We identified a protein degradation pathway at the INM in yeast mediated by the Asi complex. The Asi complex functions together with the ubiquitin-conjugating enzymes Ubc6 and Ubc7 to degrade soluble and integral membrane proteins. Genetic evidence suggests that the Asi ubiquitin ligase defines a pathway distinct from, but complementary to, ERAD. Using unbiased screening with a novel genome-wide yeast library based on a tandem fluorescent protein timer, we identify more than 50 substrates of the Asi, Hrd1 and Doa10 E3 ubiquitin ligases. We show that the Asi ubiquitin ligase is involved in degradation of mislocalized integral membrane proteins, thus acting to maintain and safeguard the identity of the INM.
+ N-end rule pathway
Systematic peptide profiling reveales the logic of N-degrons
this page is under construction
+ Libraries for high throughput yeast cell biology
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Arrayed libraries: SWAT
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Pooled libraries: CASTLING
+ Light sheet microscopes
live cell imaging without bleaching (almost)
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The πSPIM microscope
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The light pad microscope