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Publications

... the fruits of our work

Selected Publications
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COVID-19 Diagnostics
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Klein S, Müller TG, Khalid D, Sonntag-Buck V, Heuser A-M, Glass B, Meurer M, Morales I, Schillak A, Freistaedter A, et al (2020) SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP. Viruses 12: 863
 
Doa Thi VL, Herbst K, Boerner K, Meurer M, Kremer LPM, Kirrmaier D, Freistaedter A, Papagiannidis D, Galmozzi C, Klein S, Chandla P, Khalid D, Miranda IP, Schnitzler P, Kraeusslich HG, Knop M, and Anders S. 2020. Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing. Science translational medicine 12: eabc7075
 
Protein homeostasis and degradation

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Kats I, Khmelinskii A, Kschonsak M, Huber F, Knieß RA, Bartosik A & Knop M (2018) Mapping Degradation Signals and Pathways in a Eukaryotic N-terminome. Mol. Cell 70: 488–501.e5

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Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus DJ, Le Dez G, Brossard A, Gunnarsson A, Barry JD, Meurer M, Kirrmaier D, Boone C, Huber W, Rabut G, Ljungdahl PO & Knop M (2014) Protein quality control at the inner nuclear membrane. Nature 516: 410–413

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Signaling and gradients

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Durrieu L, Kirrmaier D, Schneidt T, Kats I, Raghavan S, Hufnagel L, Saunders TE & Knop M (2018) Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis. Mol. Syst. Biol. 14: e8355

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Dona E, Barry JD, Valentin G, Quirin C, Khmelinskii A, Kunze A, Durdu S, Newton LR, Fernandez-Minan A, Huber W, Knop M & Gilmour D (2013) Directional tissue migration through a self-generated chemokine gradient. Nature 503: 285–289

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Maeder CI, Hink MA, Kinkhabwala A, Mayr R, Bastiaens PIH & Knop M (2007) Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat. Cell Biol. 9: 1319–1326

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Cytoskeleton and cell differentiation

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Gibeaux R, Politi AZ, Nédélec F, Antony C & Knop M (2013) Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy. Genes Dev. 27: 335–349

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ncRNAs and gene expression regulation

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Bunina D, Stefl M, Huber F, Khmelinskii A, Meurer M, Barry JD, Kats I, Kirrmaier D, Huber W & Knop M (2017) Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities. Nucleic Acids Res. 45: 11144–11158

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Huber F, Bunina D, Gupta I, Khmelinskii A, Meurer M, Theer P, Steinmetz LM & Knop M (2016) Protein Abundance Control by Non-coding Antisense Transcription. Cell Rep 15: 2625–2636

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Methods and Instruments and Resources
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Microscopes

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Capoulade J, Wachsmuth M, Hufnagel L & Knop M (2011) Quantitative fluorescence imaging of protein diffusion and interaction in living cells. Nat. Biotechnol. 29: 835–839

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Theer P, Dragneva D & Knop M (2016) πSPIM: high NA high resolution isotropic light-sheet imaging in cell culture dishes. Scientific Reports 6: 32880

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Fluorescent protein reporters

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Guan Y, Meurer M, Raghavan S, Rebane A, Lindquist JR, Santos S, Kats I, Davidson MW, Mazitschek R, Hughes TE, Drobizhev M, Knop M & Shah JV (2015) Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein. Mol. Biol. Cell 26: 2054–2066

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Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E & Knop M (2012) Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat. Biotechnol. 30: 708–714

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Resources

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Meurer M, Duan Y, Sass E, Kats I, Herbst K, Buchmuller BC, Dederer V, Huber F, Kirrmaier D, Stefl M, Van Laer K, Dick TP, Lemberg MK, Khmelinskii A, Levy ED & Knop M (2018) Genome-wide C-SWAT library for high-throughput yeast genome tagging. Nat Methods 15: 598–600

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Yofe I, Weill U, Meurer M, Chuartzman S, Zalckvar E, Goldman O, Ben-Dor S, Schütze C, Wiedemann N, Knop M, Khmelinskii A & Schuldiner M (2016) One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy. Nat Methods 13: 371–378 

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Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E & Knop M (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21: 947–962

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Knop M, Siegers K, Pereira G, Zachariae W, Winsor B, Nasmyth K & Schiebel E (1999) Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15: 963–972

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Taxis C & Knop M (2006) System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae. BioTechniques 40: 73–78

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bioRxiv
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