Selected Publications
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Protein homeostasis and degradation
Kats I, Khmelinskii A, Kschonsak M, Huber F, Knieß RA, Bartosik A & Knop M (2018) Mapping Degradation Signals and Pathways in a Eukaryotic N-terminome. Mol. Cell 70: 488–501.e5
Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus DJ, Le Dez G, Brossard A, Gunnarsson A, Barry JD, Meurer M, Kirrmaier D, Boone C, Huber W, Rabut G, Ljungdahl PO & Knop M (2014) Protein quality control at the inner nuclear membrane. Nature 516: 410–413
Signaling and gradients
Durrieu L, Kirrmaier D, Schneidt T, Kats I, Raghavan S, Hufnagel L, Saunders TE & Knop M (2018) Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis. Mol. Syst. Biol. 14: e8355
Dona E, Barry JD, Valentin G, Quirin C, Khmelinskii A, Kunze A, Durdu S, Newton LR, Fernandez-Minan A, Huber W, Knop M & Gilmour D (2013) Directional tissue migration through a self-generated chemokine gradient. Nature 503: 285–289
Maeder CI, Hink MA, Kinkhabwala A, Mayr R, Bastiaens PIH & Knop M (2007) Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat. Cell Biol. 9: 1319–1326
Cytoskeleton and cell differentiation
Gibeaux R, Politi AZ, Nédélec F, Antony C & Knop M (2013) Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy. Genes Dev. 27: 335–349
ncRNAs and gene expression regulation
Bunina D, Stefl M, Huber F, Khmelinskii A, Meurer M, Barry JD, Kats I, Kirrmaier D, Huber W & Knop M (2017) Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities. Nucleic Acids Res. 45: 11144–11158
Huber F, Bunina D, Gupta I, Khmelinskii A, Meurer M, Theer P, Steinmetz LM & Knop M (2016) Protein Abundance Control by Non-coding Antisense Transcription. Cell Rep 15: 2625–2636
Methods and Instruments and Resources
Microscopes
Capoulade J, Wachsmuth M, Hufnagel L & Knop M (2011) Quantitative fluorescence imaging of protein diffusion and interaction in living cells. Nat. Biotechnol. 29: 835–839
Theer P, Dragneva D & Knop M (2016) πSPIM: high NA high resolution isotropic light-sheet imaging in cell culture dishes. Scientific Reports 6: 32880
Fluorescent protein reporters
Guan Y, Meurer M, Raghavan S, Rebane A, Lindquist JR, Santos S, Kats I, Davidson MW, Mazitschek R, Hughes TE, Drobizhev M, Knop M & Shah JV (2015) Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein. Mol. Biol. Cell 26: 2054–2066
Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E & Knop M (2012) Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat. Biotechnol. 30: 708–714
Resources
Meurer M, Duan Y, Sass E, Kats I, Herbst K, Buchmuller BC, Dederer V, Huber F, Kirrmaier D, Stefl M, Van Laer K, Dick TP, Lemberg MK, Khmelinskii A, Levy ED & Knop M (2018) Genome-wide C-SWAT library for high-throughput yeast genome tagging. Nat Methods 15: 598–600
Yofe I, Weill U, Meurer M, Chuartzman S, Zalckvar E, Goldman O, Ben-Dor S, Schütze C, Wiedemann N, Knop M, Khmelinskii A & Schuldiner M (2016) One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy. Nat Methods 13: 371–378
Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E & Knop M (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21: 947–962
Knop M, Siegers K, Pereira G, Zachariae W, Winsor B, Nasmyth K & Schiebel E (1999) Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15: 963–972
Taxis C & Knop M (2006) System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae. BioTechniques 40: 73–78
Julia Fueller, Matthias Meurer, Konrad Herbst, Krisztina Gubicza, Bahtiyar Kurtulmus, Julia D. Knopf, Daniel Kirrmaier, Benjamin Buchmuller, Gislene Pereira, Marius K. Lemberg, Michael Knop (2018) CRISPR/Cas12a-assisted PCR tagging of mammalian genes bioRxiv 473876
Ayse Koca Caydasi, Anton Khmelinskii, Zoulfia Darieva, Bahtiyar Kurtulmus, Michael Knop, Gislene Pereira SWR1 Chromatin Remodeling Complex Prevents Mitotic Slippage during Spindle Position Checkpoint Arrest bioRxiv 749440
